Microscopy:Article Title: Method of making induced pluripotent stem cells
Article Snippet: .. DMEM (Invitrogen, cat. no. 11965-092) Fetal bovine serum, FBS (Hyclone, Thermo Scientific) KO serum replacement (KOSR; Invitrogen, cat. no. 10828-028) PBS without Ca/Mg (Hyclone, Thermo Scientific) L-Gin, 100× (Invitrogen Inc.,) Nonessential amino acid solution (NEAA) (Invitrogen Inc.,) 2-Mercaptoethanol, 1000×, 55 mM (Invitrogen Inc.,) Sodium Pyruvate, 100× (Invitrogen Inc.,) Basic fibroblast growth factor (bFGF; Invitrogen, cat. no. 13256-029) 7.5% BSA solution (wt/vol; Invitrogen, cat. no. 15260-037) Penicillin/streptomycin, 100× (Invitrogen Inc.,) 0.25% Trypsin/EDTA (Invitrogen, cat. no. 25200-056) 0.05% Trypsin/EDTA (Invitrogen, cat. no. 25300-054) Collagenase type IV (Invitrogen, cat. no. 17104-019) Attachment factor, AF 1×, (Invitrogen, cat. no. 17104-019) Equipment Inverted tissue culture microscope with phase contrast microscope, Nikon Eclipse, TS100 (5×, 10×, 20×, 40× objectives) Stereomicroscope (Nikon; SMZ-1500 or similar) Incubator, Hera cell 240i, Thermo Scientific Biosafety cabinet with aspirator for tissue culture Biosafety cabinet with aspirator for tissue culture, fitted for stereomicroscope Tissue culture centrifuge, Sorvall, Legend X1 Centrifuge, Thermo Scientific. .. Tissue culture dishes and Flasks, 100 mm, 150 mm and T-25 Tissue culture plates, 4, 6 and 12-well Filter unit: Millex-HV, PVDF, 0.45 μm, 33 mm, Millipore Inc., Conical tubes, 15 and 50 ml Water bath Isotemp (Fisher Scientific, USA) Plastic disposable transfer pipettes, 1, 5, 10 and 25 ml Glass disposable transfer pipettes, 5 ml Disposable sterile filter system (0.22 μm, 250 ml and 500 ml) Disposable syringes, 60, 30, 10 and 1 ml Hypodermic needle, 27-30G Freezing container (Nalgene Labware, cat. no. 5100) Cell lifter (Corning, cat. no. 3008) Reagent Setup Culture medium 1 (CM-1): DMEM, 10% FBS, and 1% penicillin-streptomycin Transduction medium: culture medium 2 (CM-2): DMEM, 5% FBS, 1% NEAA, and 0.5% penicillin-streptomycin.
Article Title: Constructs for making induced pluripotent stem cells
Article Snippet: .. DMEM (Invitrogen, cat. no. 11965-092) Fetal bovine serum, FBS (Hyclone, Thermo Scientific) KO serum replacement (KOSR; Invitrogen, cat. no. 10828-028) PBS without Ca/Mg (Hyclone, Thermo Scientific) L-Gin, 100× (Invitrogen Inc.,) Nonessential amino acid solution (NEAA) (Invitrogen Inc.,) 2-Mercaptoethanol, 1000×, 55 mM (Invitrogen Inc.,) Sodium Pyruvate, 100× (Invitrogen Inc.,) Basic fibroblast growth factor (bFGF; Invitrogen, cat. no. 13256-029) 7.5% BSA solution (wt/vol; Invitrogen, cat. no. 15260-037) Penicillin/streptomycin, 100× (Invitrogen Inc.,) 0.25% Trypsin/EDTA (Invitrogen, cat. no. 25200-056) 0.05% Trypsin/EDTA (Invitrogen, cat. no. 25300-054) Collagenase type IV (Invitrogen, cat. no. 17104-019) Attachment factor, AF 1×, (Invitrogen, cat. no. 17104-019) Equipment Inverted tissue culture microscope with phase contrast microscope, Nikon Eclipse, TS100 (5×, 10×, 20×, 40× objectives) Stereomicroscope (Nikon; SMZ-1500 or similar) Incubator, Hera cell 240i, Thermo Scientific Biosafety cabinet with aspirator for tissue culture Biosafety cabinet with aspirator for tissue culture, fitted for stereomicroscope Tissue culture centrifuge, Sorvall, Legend X1 Centrifuge, Thermo Scientific. .. Tissue culture dishes and Flasks, 100 mm, 150 mm and T-25 Tissue culture plates, 4, 6 and 12-well Filter unit: Millex-HV, PVDF, 0.45 nm, 33 mm, Millipore Inc., Conical tubes, 15 and 50 ml Water bath Isotemp (Fisher Scientific, USA) Plastic disposable transfer pipettes, 1, 5, 10 and 25 ml Glass disposable transfer pipettes, 5 ml Disposable sterile filter system (0.22 μm, 250 ml and 500 ml) Disposable syringes, 60, 30, 10 and 1 ml Hypodermic needle, 27-30G Freezing container (Nalgene Labware, cat. no. 5100) Cell lifter (Corning, cat. no. 3008) Reagent Setup Culture medium 1 (CM-1): DMEM, 10% FBS, and 1% penicillin-streptomycin Transduction medium: culture medium 2 (CM-2): DMEM, 5% FBS, 1% NEAA, and 0.5% penicillin-streptomycin.
Hood:Article Title: Method of making induced pluripotent stem cells
Article Snippet: .. DMEM (Invitrogen, cat. no. 11965-092) Fetal bovine serum, FBS (Hyclone, Thermo Scientific) KO serum replacement (KOSR; Invitrogen, cat. no. 10828-028) PBS without Ca/Mg (Hyclone, Thermo Scientific) L-Gin, 100× (Invitrogen Inc.,) Nonessential amino acid solution (NEAA) (Invitrogen Inc.,) 2-Mercaptoethanol, 1000×, 55 mM (Invitrogen Inc.,) Sodium Pyruvate, 100× (Invitrogen Inc.,) Basic fibroblast growth factor (bFGF; Invitrogen, cat. no. 13256-029) 7.5% BSA solution (wt/vol; Invitrogen, cat. no. 15260-037) Penicillin/streptomycin, 100× (Invitrogen Inc.,) 0.25% Trypsin/EDTA (Invitrogen, cat. no. 25200-056) 0.05% Trypsin/EDTA (Invitrogen, cat. no. 25300-054) Collagenase type IV (Invitrogen, cat. no. 17104-019) Attachment factor, AF 1×, (Invitrogen, cat. no. 17104-019) Equipment Inverted tissue culture microscope with phase contrast microscope, Nikon Eclipse, TS100 (5×, 10×, 20×, 40× objectives) Stereomicroscope (Nikon; SMZ-1500 or similar) Incubator, Hera cell 240i, Thermo Scientific Biosafety cabinet with aspirator for tissue culture Biosafety cabinet with aspirator for tissue culture, fitted for stereomicroscope Tissue culture centrifuge, Sorvall, Legend X1 Centrifuge, Thermo Scientific. .. Tissue culture dishes and Flasks, 100 mm, 150 mm and T-25 Tissue culture plates, 4, 6 and 12-well Filter unit: Millex-HV, PVDF, 0.45 μm, 33 mm, Millipore Inc., Conical tubes, 15 and 50 ml Water bath Isotemp (Fisher Scientific, USA) Plastic disposable transfer pipettes, 1, 5, 10 and 25 ml Glass disposable transfer pipettes, 5 ml Disposable sterile filter system (0.22 μm, 250 ml and 500 ml) Disposable syringes, 60, 30, 10 and 1 ml Hypodermic needle, 27-30G Freezing container (Nalgene Labware, cat. no. 5100) Cell lifter (Corning, cat. no. 3008) Reagent Setup Culture medium 1 (CM-1): DMEM, 10% FBS, and 1% penicillin-streptomycin Transduction medium: culture medium 2 (CM-2): DMEM, 5% FBS, 1% NEAA, and 0.5% penicillin-streptomycin.
Article Title: Constructs for making induced pluripotent stem cells
Article Snippet: .. DMEM (Invitrogen, cat. no. 11965-092) Fetal bovine serum, FBS (Hyclone, Thermo Scientific) KO serum replacement (KOSR; Invitrogen, cat. no. 10828-028) PBS without Ca/Mg (Hyclone, Thermo Scientific) L-Gin, 100× (Invitrogen Inc.,) Nonessential amino acid solution (NEAA) (Invitrogen Inc.,) 2-Mercaptoethanol, 1000×, 55 mM (Invitrogen Inc.,) Sodium Pyruvate, 100× (Invitrogen Inc.,) Basic fibroblast growth factor (bFGF; Invitrogen, cat. no. 13256-029) 7.5% BSA solution (wt/vol; Invitrogen, cat. no. 15260-037) Penicillin/streptomycin, 100× (Invitrogen Inc.,) 0.25% Trypsin/EDTA (Invitrogen, cat. no. 25200-056) 0.05% Trypsin/EDTA (Invitrogen, cat. no. 25300-054) Collagenase type IV (Invitrogen, cat. no. 17104-019) Attachment factor, AF 1×, (Invitrogen, cat. no. 17104-019) Equipment Inverted tissue culture microscope with phase contrast microscope, Nikon Eclipse, TS100 (5×, 10×, 20×, 40× objectives) Stereomicroscope (Nikon; SMZ-1500 or similar) Incubator, Hera cell 240i, Thermo Scientific Biosafety cabinet with aspirator for tissue culture Biosafety cabinet with aspirator for tissue culture, fitted for stereomicroscope Tissue culture centrifuge, Sorvall, Legend X1 Centrifuge, Thermo Scientific. .. Tissue culture dishes and Flasks, 100 mm, 150 mm and T-25 Tissue culture plates, 4, 6 and 12-well Filter unit: Millex-HV, PVDF, 0.45 nm, 33 mm, Millipore Inc., Conical tubes, 15 and 50 ml Water bath Isotemp (Fisher Scientific, USA) Plastic disposable transfer pipettes, 1, 5, 10 and 25 ml Glass disposable transfer pipettes, 5 ml Disposable sterile filter system (0.22 μm, 250 ml and 500 ml) Disposable syringes, 60, 30, 10 and 1 ml Hypodermic needle, 27-30G Freezing container (Nalgene Labware, cat. no. 5100) Cell lifter (Corning, cat. no. 3008) Reagent Setup Culture medium 1 (CM-1): DMEM, 10% FBS, and 1% penicillin-streptomycin Transduction medium: culture medium 2 (CM-2): DMEM, 5% FBS, 1% NEAA, and 0.5% penicillin-streptomycin.
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